XB-PHENO-25865
Xla wt + adam11 MO + MbC + CMV reporter, Pandey A et al. (2023)
| Experiment + Assay: |
Xla wt + adam11 MO + MbC + CMV reporter +
NF18 (cell proliferation assay)
|
| Manipulations: |
| Manipulation Type |
Reagent |
Target Gene |
Stage |
Treatment Area |
|
morpholino injection
|
adam11 MO1
|
adam11 (Xla)
|
NF stage 1
|
right side
|
|
lineage tracer
|
CMV renilla plasmid
|
|
NF stage 5 (16-cell)
|
dorsal animal hemisphere blastomere
|
|
lineage tracer
|
top flash reporter plasmid
|
|
NF stage 16
|
dorsal animal hemisphere blastomere
|
|
lineage tracer
|
MbC
|
|
NF stage 16
|
dorsal animal hemisphere blastomere
|
|
| Conditions: |
| Assay Type |
Assay Stages |
Antibody |
|
cell proliferation assay
|
NF stage 18
|
|
|
|
