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XB-PHENO-25837

Xtr Wt + kmt5b MO (suv4-20h1 NF4), Angerilli A et al. (2023)

Experiment + Assay: Xtr Wt + kmt5b MO (suv4-20h1 NF4) + NF35/36 (in situ hybridization)
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection kmt5b MO1 kmt5b
 NF stage 4 (8-cell) left-dorsal blastomere
Conditions:
Assay Type Assay Stages Antibody
in situ hybridization NF stage 35 and 36 to NF stage 35 and 36  
Anatomical Phenotypes: abnormal embryo morphology, abnormally decreased number of cilium in the ciliated cell, abnormal multiciliated cell morphology
Expression Phenotypes (1):
decreased amount tuba4b expression in multiciliated epidermal cell
Source: Fig. 5 A: r2c2
Article: XB-ART-59747, PubMed