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XB-PHENO-25739

Xtr Wt + ddx3x, Perfetto M et al. (2021)

Experiment + Assay: Xtr Wt + ddx3x + NF12.5 (in situ hybridization)
Background: Xtr.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection ddx3x ddx3x
 NF stage 2 (2-cell) blastomere
Conditions:
Assay Type Assay Stages Antibody
in situ hybridization NF stage 12.5  
Anatomical Phenotypes: increased size of the neural crest, increased size of the neural plate border, increased size of the presumptive midbrain-hindbrain boundary
Expression Phenotypes (3):
increased amount gbx2.1 expression in presumptive midbrain-hindbrain boundary
increased amount msx1 expression in neural plate border
increased amount snai2 expression in neural crest
Source: Fig.S.2
Article: XB-ART-57623, PubMed