XB-PHENO-23968
Xla Wt + bmp4 + CHX + animal cap explant, Lee H et al. (2020)
| Experiment + Assay: |
Xla Wt + bmp4 + CHX + animal cap explant +
NF10 (in situ hybridization)
|
| Manipulations: |
| Manipulation Type |
Reagent |
Target Gene |
Stage |
Treatment Area |
|
mRNA injection
|
bmp4 (Xla)
|
bmp4 (Xla)
|
NF stage 3 (4-cell)
|
animal blastomere
|
|
explant
|
|
|
NF stage 8
|
animal cap
|
|
chemical
|
cycloheximide
|
|
NF stage 8
|
animal cap
|
|
| Conditions: |
| Assay Type |
Assay Stages |
Antibody |
|
in situ hybridization
|
NF stage 10
to NF stage 10
|
|
|
|
|
Expression Phenotypes (1):
|
|
increased amount
szl.L
expression in animal cap
|
|