Xenbase Image ID: 133723

Fig. 2. Spatiotemporal expression pattern of Xenopus nuclear GTP-binding protein (XGB). (A) Ribonuclease protection analysis of XGB transcripts in developing Xenopus embryos. Total RNA from embryos of stages 0–27 was hybridized with an antisense RNA probe for XGB and then subjected to ribonuclease protection analysis (lanes 0–27). XGB mRNA was first detected at stage 13 and increased towards stage 27. In lane Y, the RNA probe was hybridized with yeast RNA, and in lane C, the RNA probe was not digested with ribonuclease. (B) Whole-mount in situ hybridization showing expression of XGB in developing Xenopus embryos. B1, Dorsal view of a stage 13 embryo showing expression of XGB in the anterior neural plate. B2, Dorsal view of a stage 16 embryo showing expression of XGB in a broader area of the neural plate. B3, Dorsal view of a stage 20 embryo showing expression of XGB in the prospective eye region. B4, Lateral view of a stage 23 embryo showing expression of XGB in the optic primordium and branchial arch region. Anterior and posterior sides are indicated by A and P, respectively. Image published in: Tamanoue Y et al. (2006) Copyright © 2006. Image reproduced with permission of the Publisher. Gene Synonyms Species Stage(s) Tissue gtpbp4.L XGB X. laevis Throughout NF stage 13 neural plate cranial neural crest pre-chordal neural plate anterior neural fold gtpbp4.L XGB X. laevis Throughout NF stage 16 neural plate pre-chordal neural plate chordal neural plate gtpbp4.L XGB X. laevis Throughout NF stage 20 cranial neural crest anterior neural tube eye primordium gtpbp4.L XGB X. laevis Throughout NF stage 23 eye optic vesicle cranial neural crest neural crest Image source: Published Larger Image Printer Friendly View

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