Fig. 6. (A, B) The formation of fully activated ERK bis-phosphodimers (arrow) corresponds exactly to the measured maximum in ERK activity. Phosphorylated ERK monomers do not contribute to the extremely high ERK activity measured at 2 and especially 4Â min AF. (A) Control embryos. (B) U0126 inhibition at 15Â min before fertilisation. The whole-cell extracts used for measurement of ERK1 and cdk2 activities were subjected to WB using anti-dual-phosphorylated ERK (upper panels) or anti-ERK antibodies (lower panels). Equal amounts of total protein were loaded on each gel (see actin loading control). Times AF and molecular markers are shown. Note that highly sensitive ECL was used for active ERK-dimer visualisation (arrow). Non-denaturing gel was used to maintain ERK as a dimer.
Image published in: Kisielewska J et al. (2009)
Image downloaded from an Open Access article in PubMed Central. © 2009 Elsevier Inc.
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