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Summary Attributions Wiki
XB-GRNA-29231032
gRNA Name: rpe65 gRNA1
Synonyms:
Genomic Target: rpe65

Sequence: 5' GGGGAACTGAACAACAACCT 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 18/20 20/20 20/20
genome
gRNA
 
5' CGGTTGTTGTTCAGTTTCCC 3'
    ||||||||||||||| |||
3' TCCAACAACAAGTCAAGGGG 5'
 
5' AGGTTGTTGTTCAGTTCCCC 3'
   ||||||||||||||||||||
3' TCCAACAACAAGTCAAGGGG 5'
 
5' AGGTTGTTGTTCAGTTCCCC 3'
   ||||||||||||||||||||
3' TCCAACAACAAGTCAAGGGG 5'
    
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr4L:77979744..77979763 20/20 Antisense target rpe65.L
Chr4S:63989261..63989280 20/20 Antisense target rpe65.S
Chr2S:77187153..77187172 18/20 Antisense off-target grik3.S
Chr5L:52516547..52516566 18/20 Antisense off-target ahctf1.L
Chr1L:162864975..162864994 16/20 Antisense off-target ttc28.L
Chr5L:41054174..41054193 18/20 Antisense unknown
Chr6S:127062552..127062571 16/20 Sense unknown
Chr1L:149529366..149529385 16/20 Sense unknown
tropicalis 10.0 Chr4:83246314..83246333 18/20 Antisense target rpe65
Chr7:107468527..107468546 18/20 Antisense off-target cic
Chr5:65442749..65442768 18/20 Antisense off-target pkib
Chr10:11812744..11812763 18/20 Sense off-target skap1
Chr9:67091912..67091931 18/20 Sense off-target rapgef4
Chr5:104876492..104876511 17/20 Antisense off-target ccdc50
Chr6:109578152..109578171 18/20 Antisense unknown
Chr7:76457714..76457733 17/20 Antisense unknown
Chr9:59532499..59532518 16/20 Sense unknown
Publications
???morpholino.displaySummary.papersFirst??? RPE65 knockout Xenopus laevis have a compromised but detectable electroretinogram and altered visual responses, without retinal degeneration or altered melanophore dispersion., Exp Eye Res 2025     
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