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Summary Attributions Wiki
XB-GRNA-29213000
gRNA Name: wnt4 gRNA1
Synonyms: Wnt4 sgRNA
Genomic Target: wnt4

PAM Sequence: GTT
Sequence: 5' GGGTGCTCAGTTGGCCATAG 3'



Genomic Target laevis.L laevis.S
Identities 19/20 19/20
genome
gRNA
 
5' CTATGGCCAACTGAGCACCT 3'
   ||||||||||||||||||| 
3' GATACCGGTTGACTCGTGGG 5'
 
5' CTATGGCCAACTGAGCACCT 3'
   ||||||||||||||||||| 
3' GATACCGGTTGACTCGTGGG 5'
     
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr7L:98199047..98199066 19/20 Sense target wnt4.L
Chr7S:82445936..82445955 19/20 Sense target wnt4.S
Chr3L:49106331..49106350 19/20 Antisense off-target sh3pxd2b.L
Chr5S:110320113..110320132 18/20 Antisense off-target clstn2.S
Chr1L:79550457..79550476 16/20 Antisense off-target tspan5.L
Chr2L:141890759..141890778 18/20 Antisense unknown
Chr4S:84613627..84613646 17/20 Antisense unknown
Chr3S:111904013..111904032 16/20 Antisense unknown
Chr1S:60272817..60272836 16/20 Antisense unknown
tropicalis 10.0 Chr4:79898461..79898480 18/20 Sense off-target ptgfr
Chr6:131890501..131890520 18/20 Sense off-target rims2
Chr6:131893196..131893215 18/20 Sense off-target rims2
Chr4:54506022..54506041 17/20 Sense off-target LOC101733232
Publications
???morpholino.displaySummary.papersFirst??? Wnt4 and ephrinB2 instruct apical constriction via Dishevelled and non-canonical signaling., Nat Commun 2023     
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