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Summary Attributions Wiki
XB-GRNA-29209414
gRNA Name: sox2 gRNA4
Synonyms:
Genomic Target: sox2

Sequence: 5' CTGACAGCCCACATCACATGGACGGTTGTCTATTAACTTGTTTCAAAAAAGTAGCAGGACT TGTCACAAGCGCAGGGAGGGACAGCAGTTGGCTCAGCTGCCCGAGAGCAGAAACCGAGAGAGAGAGAGAGGCTGTGGCGGGAGAGAGAAA GTGAAGATAGTTTGGGAAACTGTGGGAAACTGATTCCGCTCTGCCAGCCTTTGCTCCCCACACGCCG 3'



Genomic Target tropicalis laevis.L
Identities 61/220 61/220
genome
gRNA
 
5' AGTCCTGCTACTTTTTTGAAACAAGTTAATAGACAACCGTCCATGTGATGTGGGCTGTCAG 3'
   |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3' TCAGGACGATGAAAAAACTTTGTTCAATTATCTGTTGGCAGGTACACTACACCCGACAGTC 5'
 
5' AGTCCTGCTACTTTTTTGAAACAAGTTAATAGACAACCGTCCATGTGATGTGGGCTGTCAG 3'
   |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
3' TCAGGACGATGAAAAAACTTTGTTCAATTATCTGTTGGCAGGTACACTACACCCGACAGTC 5'
     
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr5L:114960723..114960783 61/61 Antisense target sox2.L
Chr5S:94687104..94687164 60/61 Antisense off-target LOC108718087
tropicalis 10.0 Chr5:108037628..108037688 61/61 Antisense target sox2
Publications
???morpholino.displaySummary.papersFirst??? A CRISPR-Cas9-mediated versatile method for targeted integration of a fluorescent protein gene to visualize endogenous gene expression in Xenopus laevis., Dev Biol 2024     
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