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Summary Attributions Wiki
XB-GRNA-23659125
gRNA Name: ush2a gRNA2
Synonyms: ush2a_g1
Genomic Target: ush2a

Sequence: 5' GAGAGTCCAGTTCAGATAGA 3'



Genomic Target tropicalis
Identities 20/20
genome
gRNA
 
5' TCTATCTGAACTGGACTCTC 3'
   ||||||||||||||||||||
3' AGATAGACTTGACCTGAGAG 5'
      
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr7L:46941216..46941235 18/20 Sense off-target grid1.L
Chr4L:28154195..28154214 16/20 Antisense off-target cskmt.L
Chr1L:209008905..209008924 16/20 Antisense off-target XB5962685.L
Chr9_10S:106212271..106212290 19/20 Sense unknown
Chr4L:116627946..116627965 18/20 Antisense unknown
Chr3S:111399466..111399485 17/20 Sense unknown
Chr1S:144787138..144787157 16/20 Antisense unknown
Chr3S:123278586..123278605 16/20 Antisense unknown
tropicalis 10.0 Chr5:12795670..12795689 20/20 Antisense target ush2a
Chr1:91291516..91291535 17/20 Sense off-target kdm4b
Chr5:87158733..87158752 17/20 Antisense off-target htr1e
Chr4:130178653..130178672 16/20 Antisense unknown
Publications
???morpholino.displaySummary.papersFirst??? Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in Xenopus and zebrafish embryos., Sci Rep 2020     
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