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Summary Attributions Wiki
XB-GRNA-23659098
gRNA Name: adprhl1 gRNA12
Synonyms: -e7-2L
Genomic Target: adprhl1

PAM Sequence: TGG
Sequence: 5' GGTGTCTGTATGGATTGCTCTA 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 21/22 22/22 21/22
genome
gRNA
 
5' TAGAGCAATCCATAAAGACACC 3'
   |||||||||||||| |||||||
3' ATCTCGTTAGGTATGTCTGTGG 5'
 
5' TAGAGCAATCCATACAGACACC 3'
   ||||||||||||||||||||||
3' ATCTCGTTAGGTATGTCTGTGG 5'
 
5' TAGAGCAATCCATAAAGACACC 3'
   |||||||||||||| |||||||
3' ATCTCGTTAGGTATGTCTGTGG 5'
    
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr2L:120477625..120477646 22/22 Sense target adprhl1.L
Chr2S:106633829..106633850 21/22 Sense target adprhl1.S
Chr3S:56522219..56522240 20/22 Antisense off-target fam189a1.S
Chr4L:85913780..85913801 20/22 Sense off-target LOC108714274
Chr9_10S:11463934..11463955 19/22 Sense off-target dok5.S
Chr4S:91610904..91610925 19/22 Antisense off-target kcnt2.S
Chr9_10L:82593470..82593491 19/22 Sense off-target LOC108701415
Chr1L:108559285..108559306 18/22 Sense off-target efna2.L
Chr1S:151892778..151892799 18/22 Sense off-target hectd4.S
Chr7L:37673141..37673162 18/22 Antisense off-target marchf8.L
Chr7S:29324339..29324360 18/22 Antisense off-target marchf8.S
Chr5L:61912934..61912955 19/22 Antisense unknown
Chr6L:68425017..68425038 19/22 Sense unknown
Chr3S:34924707..34924728 19/22 Antisense unknown
Chr3S:99915715..99915736 19/22 Antisense unknown
Chr6L:97411584..97411605 18/22 Antisense unknown
Chr3S:24168609..24168630 18/22 Sense unknown
tropicalis 10.0 Chr2:115908706..115908727 21/22 Sense target adprhl1
Chr4:262136..262157 18/22 Sense off-target LOC101734942
Chr4:283729..283750 18/22 Sense off-target LOC101734942
Chr9:23206880..23206901 18/22 Sense off-target LOC116407726
Chr2:11674300..11674321 19/22 Sense unknown
Chr3:64613910..64613931 19/22 Antisense unknown
Chr4:54584431..54584452 19/22 Antisense unknown
Chr8:11944011..11944032 19/22 Sense unknown
Publications
???morpholino.displaySummary.papersFirst??? Defective heart chamber growth and myofibrillogenesis after knockout of adprhl1 gene function by targeted disruption of the ancestral catalytic active site., PLoS One 2020     
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