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Antibody Name: Mcm7 Ab3 Common Name: RRID: Synonyms: Antigens Clone Type: Polyclonal Source:

Antibody
Clone Number
Purification
Isotype	isotype unknown
Host Organism	rabbit
Xenopus Reactivity	X. laevis
Non-Xenopus Reactivity
Description:	Generated in Ronald A. Laskey lab, 1996 Full-length XMCM7 and C-terminal fragment (545-720) of XMCM7 were cloned as SphI-BamHI fragments into pQE70 His-tag expression vector (Qiagen, Chatsworth, CA). Both recombinant polypeptides were expressed in E.coli and purified on Ni2+-NTA agarose (Qiagen). C-terminal fragment of XMCM7 was injected into rabbits. Full-length XMCM7 was immobilized on SulfolinkGel (Pierce) and used to affinity purify the antibodies.
Immunogen
Name
Type	polypeptide
Post Translational Modifications	None
Source Organism	frog
Description
Sequence
Reported Usage
Publications
???displayAntibody.papersFirst???	XMCM7, a novel member of the Xenopus MCM family, interacts with XMCM3 and colocalizes with it throughout replication., Proc Natl Acad Sci U S A 1996
???displayAntibody.papersRecent???	Rif1 restrains the rate of replication origin firing in Xenopus laevis., Commun Biol 2023
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