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Antibody Name: Hist1H3A Ab2 Common Name: RRID: AB_2616029 Synonyms: 9733, H3/A, H3FA Antigens Clone Type: Monoclonal Source: Cell Signaling Technology

Antibody
Clone Number
Purification
Isotype	IgG
Host Organism	rabbit
Xenopus Reactivity	X. tropicalis, X. laevis
Non-Xenopus Reactivity
Description:	Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb detects endogenous levels of histone H3 only when tri-methylated on Lys27. The antibody does not cross-react with non-methylated, mono-methylated or di-methylated Lys27. In addition, the antibody does not cross-react with mono-methylated, di-methylated or tri-methylated histone H3 at Lys4, Lys9, Lys36 or Histone H4 at Lys20. NOn-mamalian species predicted to react based on 100% sequence homology: Xenopus, Zebrafish
Immunogen
Name	Histone H3
Type	polypeptide
Post Translational Modifications	Methylated
Source Organism	rabbit
Description	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of histone H3 in which Lys27 is tri-methylated.
Sequence
Reported Usage
immunohistochemistry	1:250
Publications
???displayAntibody.papersFirst???	Gene Resistance to Transcriptional Reprogramming following Nuclear Transfer Is Directly Mediated by Multiple Chromatin-Repressive Pathways., Mol Cell 2017
???displayAntibody.papersRecent???	Chromatin accessibility analysis reveals distinct functions for HDAC and EZH2 activities in early appendage regeneration., Wound Repair Regen 2022
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