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Malays J Med Sci
2018 Feb 01;251:101-113. doi: 10.21315/mjms2018.25.1.12.
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The Effects of 4-Hydroxybenzoic Acid Identified from Bamboo (Dendrocalamus asper) Shoots on Kv1.4 Channel.
Zhang J
,
Mohamad FH
,
Wong JH
,
Mohamad H
,
Ismail AH
,
Mohamed Yusoff AA
,
Osman H
,
Wong KT
,
Idris Z
,
Abdullah JM
.
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BACKGROUND: Bamboo shoot has been used as a treatment for epilepsy in traditional Chinese medicine for generations to treat neuronal disorders such as convulsive, dizziness and headaches. 4-hydroxybenzoic acid (4-hba) is a non-flavonoid phenol found abundantly in Dendrocalamus asper shoots (bamboo), fruits (strawberries and apples) and flowers. Kv1.4 is a rapidly inactivating Shaker-related member of the voltage-gated potassium channels with two inactivation mechanisms; the fast N-type and slow C-type. It plays vital roles in repolarisation, hyperpolarisation and signaling the restoration of resting membrane potential through the regulation of the movement of K+ across the cellular membrane.
METHODS: Chemical compounds from Dendrocalamus asper bamboo shoots were purified and identified as major palmitic acids mixed with other minor fatty acids, palmitic acid, 4-hydroxybenzaldehyde, lauric acid, 4-hydroxybenzoic acid and cholest-4-ene-3-one. The response of synthetic 4-hydroxybenzoic acid was tested on Kv1.4 potassium channel which was injected into viable oocytes that was extracted from Xenopus laevis. The current were detected by the two-microelectrode voltage clamp, holding potential starting from -80 mV with 20 mV step-up until +80 mV. Readings of treatments with 0.1% DMSO, 4-hba concentrations and K channel blockers were taken at +60 mV. The ratio of tail/peak amplitude is the index of the activity of the Kv1.4 channels with n ≥ 6 (number of oocytes tested). The decreases of the ratios of five different concentrations (1 μM, 10 μM, 100 μM, 1 mM and 2.5 mM) were compared with 0.1% DMSO as the control.
RESULTS: All concentration showed statistically significant results with P < 0.05 except for 100 μM. The normalised current of the 4-hba concentrations were compared with potassium channel blockers (TEA and 4-AP) and all groups showed statistically significant results. This study also showed that time taken for each concentration to affect Kv1.4 does not play any significant roles.
CONCLUSION: 4-hydroxybenzoic acid was found to be able to enhance the inactivation of Kv1.4 by lowering the membrane potential so that the abnormal neuronal firing can be inhibited. With IC50 slightly higher than 10 μM, increasing concentrations (100 μM, 1 mM and 2.5 mM) had shown to exhibit toxicity effects. The best concentration from this study is 10 μM with Hill slope of 0.1799.
Abdullah,
Interesting asian plants: their compounds and effects on electrophysiology and behaviour.
2011, Pubmed
Abdullah,
Interesting asian plants: their compounds and effects on electrophysiology and behaviour.
2011,
Pubmed
Barros,
Cytoplasmic domains and voltage-dependent potassium channel gating.
2012,
Pubmed
Bett,
Inactivation and recovery in Kv1.4 K+ channels: lipophilic interactions at the intracellular mouth of the pore.
2004,
Pubmed
,
Xenbase
Bezanilla,
Negative conductance caused by entry of sodium and cesium ions into the potassium channels of squid axons.
1972,
Pubmed
Chanda,
A common pathway for charge transport through voltage-sensing domains.
2008,
Pubmed
Chen,
Acceleration of P/C-type inactivation in voltage-gated K(+) channels by methionine oxidation.
2000,
Pubmed
,
Xenbase
Chindo,
Anticonvulsant properties of saponins from Ficus platyphylla stem bark.
2009,
Pubmed
Ciorba,
Modulation of potassium channel function by methionine oxidation and reduction.
1997,
Pubmed
,
Xenbase
Claydon,
K+ activation of kir3.1/kir3.4 and kv1.4 K+ channels is regulated by extracellular charges.
2004,
Pubmed
,
Xenbase
D'Adamo,
K(+) channelepsy: progress in the neurobiology of potassium channels and epilepsy.
2013,
Pubmed
Dascal,
Inhibition of function in Xenopus oocytes of the inwardly rectifying G-protein-activated atrial K channel (GIRK1) by overexpression of a membrane-attached form of the C-terminal tail.
1995,
Pubmed
,
Xenbase
González-Pérez,
Slow inactivation in Shaker K channels is delayed by intracellular tetraethylammonium.
2008,
Pubmed
,
Xenbase
Gutman,
International Union of Pharmacology. LIII. Nomenclature and molecular relationships of voltage-gated potassium channels.
2005,
Pubmed
Hoshi,
C-type inactivation of voltage-gated K+ channels: pore constriction or dilation?
2013,
Pubmed
Imbrici,
Episodic ataxia type 1 mutations affect fast inactivation of K+ channels by a reduction in either subunit surface expression or affinity for inactivation domain.
2011,
Pubmed
,
Xenbase
Jiang,
C-type inactivation involves a significant decrease in the intracellular aqueous pore volume of Kv1.4 K+ channels expressed in Xenopus oocytes.
2003,
Pubmed
,
Xenbase
Laura,
Analysis of connexin expression during seizures induced by 4-aminopyridine in the rat hippocampus.
2015,
Pubmed
Lee,
The effects of ginsenoside Rg(3) on human Kv1.4 channel currents without the N-terminal rapid inactivation domain.
2009,
Pubmed
,
Xenbase
Li,
Regulation of N- and C-type inactivation of Kv1.4 by pHo and K+: evidence for transmembrane communication.
2003,
Pubmed
,
Xenbase
McAllister,
The time and voltage dependence of the slow outward current in cardiac Purkinje fibres.
1966,
Pubmed
Muazu,
A review of traditional plants used in the treatment of epilepsy amongst the Hausa/Fulani tribes of northern Nigeria.
2008,
Pubmed
Muceniece,
Betulin binds to gamma-aminobutyric acid receptors and exerts anticonvulsant action in mice.
2008,
Pubmed
Nassiri-Asl,
Anticonvulsive effects of intracerebroventricular administration of rutin in rats.
2008,
Pubmed
Nongdam,
The Nutritional Facts of Bamboo Shoots and Their Usage as Important Traditional Foods of Northeast India.
2014,
Pubmed
Nsour,
Review on phytotherapy in epilepsy.
2000,
Pubmed
O'Connell,
Xenopus laevis oocytes infected with multi-drug-resistant bacteria: implications for electrical recordings.
2011,
Pubmed
,
Xenbase
Oliva,
Slow inactivation in voltage gated potassium channels is insensitive to the binding of pore occluding peptide toxins.
2005,
Pubmed
,
Xenbase
Ponce,
Voltage-dependent potassium currents expressed in Xenopus laevis oocytes after injection of mRNA isolated from trophozoites of Giardia lamblia (strain Portland-1).
2013,
Pubmed
,
Xenbase
Rasmusson,
Bi-stable block by 4-aminopyridine of a transient K+ channel (Kv1.4) cloned from ferret ventricle and expressed in Xenopus oocytes.
1995,
Pubmed
,
Xenbase
Rodriguez-Menchaca,
PIP2 controls voltage-sensor movement and pore opening of Kv channels through the S4-S5 linker.
2012,
Pubmed
,
Xenbase
Sahoo,
Oxidative modulation of voltage-gated potassium channels.
2014,
Pubmed
Sahranavard,
Medicinal plants used in Iranian traditional medicine to treat epilepsy.
2014,
Pubmed
Simić,
Electrochemical behavior and antioxidant and prooxidant activity of natural phenolics.
2007,
Pubmed
Sobczak,
Endogenous transport systems in the Xenopus laevis oocyte plasma membrane.
2010,
Pubmed
,
Xenbase
Sun,
Coumarins from the leaves of Bambusa pervariabilis McClure.
2010,
Pubmed
Terhag,
Cave Canalem: how endogenous ion channels may interfere with heterologous expression in Xenopus oocytes.
2010,
Pubmed
,
Xenbase
Trimmer,
Localization of voltage-gated ion channels in mammalian brain.
2004,
Pubmed
Wang,
Kv1.4 channel block by quinidine: evidence for a drug-induced allosteric effect.
2003,
Pubmed
,
Xenbase
Weber,
Endogenous ion channels in oocytes of xenopus laevis: recent developments.
1999,
Pubmed
,
Xenbase
Wulff,
Voltage-gated potassium channels as therapeutic targets.
2009,
Pubmed
Xie,
Synaptotagmin I delays the fast inactivation of Kv1.4 channel through interaction with its N-terminus.
2014,
Pubmed
Zhu,
Medicinal compounds with antiepileptic/anticonvulsant activities.
2014,
Pubmed
