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Biophys J
2011 Jun 08;10011:2810-9. doi: 10.1016/j.bpj.2011.03.047.
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Single-shot optical sectioning using two-color probes in HiLo fluorescence microscopy.
Muro E
,
Vermeulen P
,
Ioannou A
,
Skourides P
,
Dubertret B
,
Fragola A
,
Loriette V
.
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We describe a wide-field fluorescence microscope setup which combines HiLo microscopy technique with the use of a two-color fluorescent probe. It allows one-shot fluorescence optical sectioning of thick biological moving sample which is illuminated simultaneously with a flat and a structured pattern at two different wavelengths. Both homogenous and structured fluorescence images are spectrally separated at detection and combined similarly with the HiLo microscopy technique. We present optically sectioned full-field images of Xenopus laevis embryos acquired at 25 images/s frame rate.
