Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Hypotheses on general olfaction can be divided into two broad groups: those that predict the existence of olfactory-specific olfactory receptor proteins and those that do not. Recently, much attention has been paid to the discovery of an odorant-stimulated adenylate cyclase in purified olfactory cilia. This finding has, for the most part, been accepted as evidence that the former hypotheses are correct. Here we report that frog melanophores, which are nonolfactory in nature, disperse their melanosomes in response to the same types and concentrations of odorants used in the investigations of olfactory cilia and that pigment dispersion is accompanied by rises in intracellular cAMP levels. The effects show that the existence of a cAMP-based second messenger system in olfactory cilia is not in itself proof of the existence of olfactory-specific olfactory receptor proteins. Also they explain the basis of Ottoson's pioneering work of 30 years ago on the electrical responses of frog olfactory epithelium to stimulation with alcohols. The results suggest that there could be two mechanisms that are important for the detection of odorants: one based on specific receptors, the other nonspecific, but both working through activation of cAMP.
Anholt,
Transduction proteins of olfactory receptor cells: identification of guanine nucleotide binding proteins and protein kinase C.
1987, Pubmed
Anholt,
Transduction proteins of olfactory receptor cells: identification of guanine nucleotide binding proteins and protein kinase C.
1987,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Cagan,
Biochemical studies of olfaction: binding specificity of radioactively labeled stimuli to an isolated olfactory preparation from rainbow trout (Salmo gairdneri).
1978,
Pubmed
Chen,
Membrane proteins unique to vertebrate olfactory cilia: candidates for sensory receptor molecules.
1984,
Pubmed
Chen,
Polypeptide gp95. A unique glycoprotein of olfactory cilia with transmembrane receptor properties.
1986,
Pubmed
Fesenko,
Molecular mechanisms of olfactory reception. IV. Some biochemical characteristics of the camphor receptor from rat olfactory epithelium.
1979,
Pubmed
Kashiwayanagi,
Evidence for non-receptor odor discrimination using neuroblastoma cells as a model for olfactory cells.
1985,
Pubmed
Kondo,
Long-term cultivation of amphibian melanophores. In vitro ageing and spontaneous transformation to a continuous cell line.
1983,
Pubmed
LERNER,
Pigment cell regulatory factors.
1959,
Pubmed
Luthin,
Activation of adenylate cyclase by alcohols requires the nucleotide-binding protein.
1984,
Pubmed
Nakamura,
A cyclic nucleotide-gated conductance in olfactory receptor cilia.
,
Pubmed
OTTOSON,
Studies on the relationship between olfactory stimulating effectiveness and physico-chemical properties of odorous compounds.
1958,
Pubmed
Pace,
Odorant-sensitive adenylate cyclase may mediate olfactory reception.
,
Pubmed
Persaud,
Analysis of discrimination mechanisms in the mammalian olfactory system using a model nose.
1982,
Pubmed
Pevsner,
Odorant-binding protein: localization to nasal glands and secretions.
1986,
Pubmed
Pevsner,
Isolation and characterization of an olfactory receptor protein for odorant pyrazines.
1985,
Pubmed
Rhein,
Biochemical studies of olfaction: binding specificity of odorants to a cilia preparation from rainbow trout olfactory rosettes.
1983,
Pubmed
Sklar,
The odorant-sensitive adenylate cyclase of olfactory receptor cells. Differential stimulation by distinct classes of odorants.
1986,
Pubmed
Steiner,
Radioimmunoassay for cyclic nucleotides. I. Preparation of antibodies and iodinated cyclic nucleotides.
1972,
Pubmed
