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ECB-GRNA-26396871

gRNA Name: fzd2 gRNA3
Synonyms: Pm_Fz1/2/7 200
Genomic Target: fzd2
Derived from:

Sequence: 5' CAGACAGGAGGAAGCCGGCT 3'



Genomic Target P. miniata
Identities 20/20
genome
gRNA
 
5' AGCCGGCTTCCTCCTGTCTG 3'
   ||||||||||||||||||||
3' TCGGCCGAAGGAGGACAGAC 5'
      
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145611.1:15934733..15934752 18/20 Antisense off-target pola2
NW_022145602.1:14034745..14034764 18/20 Antisense off-target LOC753825
NW_022145611.1:462196..462215 18/20 Sense off-target LOC754884
NW_022145603.1:18492310..18492329 16/20 Sense off-target tm7sf3
NW_022145612.1:35037220..35037239 18/20 Sense unknown
NW_022145601.1:806355..806374 17/20 Antisense unknown
P. miniata 3.0 NW_024037054.1:6174888..6174907 20/20 Sense target fzd2
NW_024037051.1:6524740..6524759 17/20 Sense off-target recql
NW_024037072.1:4719304..4719323 18/20 Antisense unknown
L. variegatus 3.0 chr13:34718887..34718906 16/20 Antisense off-target LOC121426561
Publications
???morpholino.displaySummary.papersFirst??? Molecular mechanisms of tubulogenesis revealed in the sea star hydro-vascular organ., Nat Commun 2023  
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