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Echinobase

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ECB-GRNA-26396866

gRNA Name: fgfr3 gRNA1
Synonyms: Pm-FGFR1 31
Genomic Target: fgfr3
Derived from:

Sequence: 5' GGTCACAGAACTGAAGAAAG 3'



Genomic Target P. miniata
Identities 19/20
genome
gRNA
5' CTTTCTTCAGTTCTGTGATC 3'
   |||||||||||||||||| |
3' GAAAGAAGTCAAGACACTGG 5'
     
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145545.1:304848..304867 18/20 Sense off-target LOC753458
NW_022145612.1:52671965..52671984 18/20 Sense off-target LOC584092
NW_022145612.1:53189671..53189690 18/20 Sense off-target LOC115918090
NW_022145605.1:12515161..12515180 17/20 Antisense off-target nup88
NW_022145612.1:1521745..1521764 17/20 Sense off-target LOC586628
NW_022145613.1:7497030..7497049 17/20 Antisense off-target LOC592650
NW_022145598.1:12130838..12130857 17/20 Sense off-target LOC591372
NW_022145594.1:1376465..1376484 17/20 Sense off-target LOC105441149
NW_022145596.1:24667411..24667430 16/20 Antisense off-target thoc2
NW_022145606.1:17657174..17657193 16/20 Antisense off-target pkmyt1
NW_022145614.1:19010067..19010086 16/20 Sense off-target st3gal2
NW_022145604.1:8461758..8461777 16/20 Antisense off-target LOC579068
NW_022145595.1:16738456..16738475 16/20 Sense off-target LOC105444727
NW_022145600.1:18833783..18833802 16/20 Antisense off-target LOC105438490
NW_022145600.1:18934553..18934572 16/20 Antisense off-target LOC105445336
NW_022145594.1:38107258..38107277 18/20 Sense unknown
NW_022145612.1:12302427..12302446 17/20 Sense unknown
NW_022145612.1:12883232..12883251 17/20 Sense unknown
NW_022145599.1:7521673..7521692 17/20 Sense unknown
NW_022145606.1:4041777..4041796 16/20 Antisense unknown
NW_022145603.1:18604875..18604894 16/20 Sense unknown
P. miniata 3.0 NW_024037076.1:23054482..23054501 19/20 Antisense target fgfr3
NW_024037071.1:2337978..2337997 18/20 Antisense off-target plce1
NW_024037047.1:9352651..9352670 18/20 Antisense off-target LOC119722373
NW_024037069.1:19521565..19521584 17/20 Antisense off-target LOC119738603
NW_024037074.1:34136516..34136535 16/20 Antisense off-target pdzd2
NW_024037055.1:3525780..3525799 16/20 Sense off-target LOC119723500
NW_024037063.1:7162954..7162973 16/20 Sense off-target LOC119731866
NW_024037074.1:27167635..27167654 16/20 Antisense off-target LOC119745066
NW_024037055.1:13436806..13436825 16/20 Antisense off-target LOC119724045
NW_024037055.1:14907652..14907671 16/20 Antisense off-target LOC119723937
NW_024037068.1:2380243..2380262 18/20 Sense unknown
NW_024037076.1:20127678..20127697 17/20 Sense unknown
L. variegatus 3.0 chr9:24589658..24589677 18/20 Antisense off-target LOC121421621
chr16:27594454..27594473 18/20 Antisense off-target LOC121430107
chr16:28069227..28069246 18/20 Sense off-target LOC121430042
chr8:32131165..32131184 17/20 Sense off-target LOC121419650
chr10:14929655..14929674 17/20 Sense off-target LOC121422212
chr10:33439567..33439586 17/20 Sense off-target LOC121423155
chr5:8358647..8358666 16/20 Antisense off-target znf687bl
chr3:4810924..4810943 16/20 Sense off-target LOC121410043
chr5:44892488..44892507 16/20 Sense off-target LOC121416265
chr5:46307186..46307205 16/20 Sense off-target LOC121416295
chr2:35046883..35046902 16/20 Antisense off-target LOC121406787
chr17:20930759..20930778 17/20 Sense unknown
chr13:18350158..18350177 16/20 Sense unknown
chr14:5069464..5069483 16/20 Sense unknown
chr16:23467220..23467239 16/20 Antisense unknown
chr16:24279656..24279675 16/20 Antisense unknown
Publications
???morpholino.displaySummary.papersFirst??? Molecular mechanisms of tubulogenesis revealed in the sea star hydro-vascular organ., Nat Commun 2023  
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