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Echinobase

Summary Attributions Wiki
ECB-GRNA-26396865

gRNA Name: six1 gRNA3
Synonyms: Pm-Six1/2 274
Genomic Target: six1
Derived from:

Sequence: 5' AGGCTGAGAAACTCCGGGGC 3'



Genomic Target P. miniata
Identities 20/20
genome
gRNA
 
5' GCCCCGGAGTTTCTCAGCCT 3'
   ||||||||||||||||||||
3' CGGGGCCTCAAAGAGTCGGA 5'
      
Genomic Alignments Position Identity Strand Target Genomic Target
S. purpuratus 5.0 NW_022145602.1:21182719..21182738 16/20 Antisense unknown
P. miniata 3.0 NW_024037051.1:5761323..5761342 20/20 Sense target six1
NW_024037051.1:6163958..6163977 18/20 Sense off-target LOC119721019
NW_024037051.1:6215356..6215375 18/20 Sense off-target LOC119721027
NW_024037069.1:19430328..19430347 17/20 Sense off-target bop1
NW_024037047.1:2112018..2112037 17/20 Sense off-target LOC119736960
XR_005272332.1:571..590 17/20 off-target LOC119736960
NW_024037047.1:2118451..2118470 17/20 Sense unknown
NW_024037047.1:2132111..2132130 17/20 Sense unknown
NW_024037076.1:10486560..10486579 17/20 Sense unknown
Publications
???morpholino.displaySummary.papersFirst??? Molecular mechanisms of tubulogenesis revealed in the sea star hydro-vascular organ., Nat Commun 2023  
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