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ECB-ART-53391
Sci Data 2024 Nov 18;111:1245. doi: 10.1038/s41597-024-03985-8.
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Chromosome-level genome assembly and annotation of the tropical sea cucumber Stichopus monotuberculatus.

Chen T , Yang Y , Wang X , Qin Z , Xie Z , Fan D , Ren C , Sun H , Luo P , Jiang X , Long H , Chen C , Pan W , E Z , Huang J , Huang Q , Xu J , Zhang Z , Cheng C , Yu S , Wang Y , Jiang F , Yan A , Hu C .


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In this study, a chromosome-level genome of the tropical sea cucumber Stichopus monotuberculatus was generated by a combination of Nanopore long-read, Illumina short-read, and Hi-C sequencing technologies. The final assembly was 810.54 Mb in length, with contig N50 and scaffold N50 values of 10.15 Mb and 35.36 Mb, respectively. This assembly comprised 23 pseudo-chromosomes, covering 99.82% of the genome. Completeness analysis using BUSCO indicated that 97.8% of the metazoan conserved genes were presented in their entirety. A total of 29,596 protein-coding genes were predicted, with functional annotations available for 94.43% of these genes. The high-quality genome assembly produced in this study may provide an essential foundation for future researches on resource conservation and genetic breeding of S. monotuberculatus.

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