ECB-ART-52013
Dev Growth Differ
1974 Jan 01;161:67-74. doi: 10.1111/j.1440-169X.1974.00067.x.
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INITIATION OF CLEAVAGE IN FISH EGGS BY INJECTION OF FLAGELLA OR MICROTUBULES OF SEA URCHIN SPERMATOZOA.
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Flagella and their microtubules obtained from sea urchin (Hemicentrotus pulcherrimus) spermatozoa were injected into unfertilized eggs of the medaka (Oryzias latipes) with a micropipette. Upon activation, some of the eggs began the first cleavage with three or more irregular blastomeres, and developed to the morula stage. It is suggested that sperm flagellar microstubule material is one of the cleavage initiation substances.
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