Dev Growth Differ 1987 Apr 01;292:161-169. doi: 10.1111/j.1440-169X.1987.00161.x.

Purification of Co-ARIS, a Cofactor for Acrosome Reaction-Inducing Substance, from the Egg Jelly of Starfish.

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In the starfish, Asterias amurensis, the acrosome reaction-inducing capacity of the egg jelly has been attributed to two components in the egg jelly: a sulfated glycoprotein (ARIS) and an unidentified low-molecular weight substance (Co-ARIS). In the process of purification of Co-ARIS, we found that Co-ARIS is not a single chemical entity but a series of closely related molecules. Co-ARIS was first group-separated by using octadecylsilane and anion-exchanger. Three major Co-ARIS' (I, II and III) were then purified by successive chromatographies using octadecylsilane and silica gel to the homogeneity in thin layer chromatography. Each of purified Co-ARIS', together with ARIS or the Pronase-digest of ARIS, induced the acrosome reaction at high ratios in normal seawater.

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