Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Proc Natl Acad Sci U S A
1983 Jul 01;8014:4484-8. doi: 10.1073/pnas.80.14.4484.
Show Gene links
Show Anatomy links
Efficient generation in vitro, from human peripheral blood cells, of monoclonal Epstein-Barr virus transformants producing specific antibody to a variety of antigens without prior deliberate immunization.
Winger L
,
Winger C
,
Shastry P
,
Russell A
,
Longenecker M
.
???displayArticle.abstract???
This paper describes a simple protocol for the efficient generation of large numbers of human monoclonal antibody-producing cells. This system is based on initial limiting-dilution culture after Epstein-Barr virus exposure of highly enriched precursors selected from peripheral blood mononuclear cells. Precursors can be enriched by using rosetting or panning approaches. Antibodies to erythrocytes, a mouse mammary carcinoma, DNA, and sperm antigens, produced without any deliberate immunization, are described. Large-scale human monoclonal antibody production may be facilitated by a combination of this protocol with a human cellular fusion system. For efficient precursor analysis and short-term (2 months or more) monoclonal antibody production, however, the system described here may be sufficient.
Brown,
Immunoglobulin expression by human B lymphocytes clonally transformed by Epstein Barr virus.
1982, Pubmed
Brown,
Immunoglobulin expression by human B lymphocytes clonally transformed by Epstein Barr virus.
1982,
Pubmed
Butler,
Delineation of optimal conditions for producing mouse-human heterohybridomas from human peripheral blood B cells of immunized subjects.
1983,
Pubmed
Clough,
Deficiency of T cell mediated regulation of anti-DNA production in systemic lupus erythematosus.
1980,
Pubmed
Crawford,
Production of human monoclonal antibody to rhesus D antigen.
1983,
Pubmed
Crawford,
Production of human monoclonal antibody to X31 influenza virus nucleoprotein.
1983,
Pubmed
Croce,
Production of human hybridomas secreting antibodies to measles virus.
1980,
Pubmed
Davis,
Analysis of DNA structure by hydroxyapatite columns and ethidium bromide fluorescence techniques. A comparative study and effect on DNA binding.
1978,
Pubmed
Edwards,
A human-hybridoma system based on a fast-growing mutant of the ARH-77 plasma cell leukemia-derived line.
1982,
Pubmed
Kaplan,
An improved rosetting assay for detection of human T lymphocytes.
1974,
Pubmed
Klotz,
An enzyme-linked immunosorbent assay for antibodies to native and denatured DNA.
1979,
Pubmed
Konowalchuk,
Immunoglobulin properties of Epstein-Barr virus transformed human umbilical cord and adult peripheral blood lymphocytes.
1982,
Pubmed
Kozbor,
Requirements for the establishment of high-titered human monoclonal antibodies against tetanus toxoid using the Epstein-Barr virus technique.
1981,
Pubmed
Kozbor,
Establishment of anti-TNP antibody-producing human lymphoid lines by preselection for hapten binding followed by EBV transformation.
1979,
Pubmed
Kozbor,
Human hybridomas constructed with antigen-specific Epstein-Barr virus-transformed cell lines.
1982,
Pubmed
Lane,
Human monoclonal anti-keyhole limpet hemocyanin antibody-secreting hybridoma produced from peripheral blood B lymphocytes of a keyhole limpet hemocyanin-immune individual.
1982,
Pubmed
Lee,
Monoclonal antibodies showing sequence specificity in their interaction with single-stranded DNAs.
1981,
Pubmed
Longenecker,
Structure and properties of the major histocompatibility complex of the chicken. Speculations on the advantages and evolution of polymorphism.
1981,
Pubmed
Longenecker,
"Natural" antibodies to chicken MHC antigens are present in mice, rats, humans, alligators and allogeneic chickens.
1980,
Pubmed
Miki,
Induction of IgG production in a human monoclonal B lymphoblastoid cell line by a B cell-specific monoclonal antibody.
1982,
Pubmed
Mosmann,
The high background immune reactivity of mice to polymorphic determinants on xenogeneic erythrocytes: theoretical and practical implications.
1982,
Pubmed
Muraguchi,
T cell-replacing factor- (TRF) induced IgG secretion in a human B blastoid cell line and demonstration of acceptors for TRF.
1981,
Pubmed
Nowell,
Proliferation, differentiation, and cytogenetics of chronic leukemic B lymphocytes cultured with mitomycin-treated normal cells.
1981,
Pubmed
Olsson,
Human-human hybridomas producing monoclonal antibodies of predefined antigenic specificity.
1980,
Pubmed
Rahman,
A monoclonal antibody specific for the Thomsen-Friedenreich cryptic T antigen.
1982,
Pubmed
Ratcliffe,
Synthesis of the t [beta-D-Gal-(1 goes to 3)-alpha-D-GalNAc]-antigenic determinant in a form useful for the preparation of an effective artificial antigen and the corresponding immunoadsorbent.
1981,
Pubmed
Shiozawa,
In vitro cooperation of a antigen-specific T cell-derived helper factor, B cells, and adherent cells or their secretory product in a primary IgM response to chicken MHC antigens.
1980,
Pubmed
Sikora,
Human hybridomas from malignant gliomas.
1982,
Pubmed
Springer,
Human carcinoma-associated precursor antigens of the blood group MN system and the host's immune responses to them.
1979,
Pubmed
Stein,
Patterns of isotype commitment in human B cells: limiting dilution analysis of Epstein Barr virus-infected cells.
1983,
Pubmed
Steinitz,
EB virus-induced B lymphocyte cell lines producing specific antibody.
1977,
Pubmed
Yarchoan,
Limiting dilution analysis of Epstein-Barr virus-induced immunoglobulin production by human B cells.
1983,
Pubmed
Yoshizaki,
Induction of proliferation and Ig production in human B leukemic cells by anti-immunoglobulins and T cell factors.
1982,
Pubmed
