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Nucleic Acids Res
1982 Mar 25;106:2093-108. doi: 10.1093/nar/10.6.2093.
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Construction of chimeric plasmids containing histone H5 cDNA from hen erythrocyte. DNA sequence of a fragment derived from the 5'' region of H5 mRNA.
Ruiz-Vazquez R
,
Ruiz-Carillo A
.
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We report that construction and characterization of chicken erythrocyte histone H5 cDNA recombinant plasmids. cDNA was synthesized from poly(A)+ polysomal RNA enriched in H5 mRNA and inserted into the PstI site of pBR322. Several clones containing H5 cDNA sequences were obtained and one of them (p541), expressing H5 antigenic determinants, was sequenced. The DNA insert of p541 contains 118 nucleotides from the 5'' non-translated region of H5 mRNA and sequences coding for up to residue 46 of the N-terminus of the arginine (position 15) H5 variant. There is a strikingly high number of repeated sequences both in the leader and coding region; among these, the octanucleotide 5'' GCG GCG GC 3'' is found five times along the sequence. Although the H5 mRNA 5'' leader is GC-rich (66%), there is an AT-rich region, about 16 nucleotides long, which shares strong homology with the leaders of sea urchin histone H1 mRNAs.
Adesnik,
Biogenesis and characterization of histone messenger RNA in HeLa cells.
1972, Pubmed
Adesnik,
Biogenesis and characterization of histone messenger RNA in HeLa cells.
1972,
Pubmed
Alwine,
Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
1977,
Pubmed
Appels,
Synthesis and turnover of DNA-bound histone during maturation of avian red blood cells.
1972,
Pubmed
Beug,
Chicken hematopoietic cells transformed by seven strains of defective avian leukemia viruses display three distinct phenotypes of differentiation.
1979,
Pubmed
Billett,
A study of the quantitative variation of histones, and their relationship to RNA synthesis, during erythropoiesis in the adult chicken.
1972,
Pubmed
Borun,
Rapidly labeled, polyribosome-associated RNA having the properties of histone messenger.
1967,
Pubmed
Briand,
Chicken erythrocyte histone H5. IV. Sequence of the carboxy-termined half of the molecule (96 residues) and complete sequence.
1980,
Pubmed
Dagert,
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
1979,
Pubmed
Gazzolo,
Early precursors in the erythroid lineage are the specific target cells of avian erythroblastosis virus in vitro.
1980,
Pubmed
Greenaway,
Heterogeneity and polymorphism in chicken erythrocyte histone fraction V.
1971,
Pubmed
Grunstein,
Messenger RNAs for individual histone proteins: fingerprint analysis and in vitro translation.
1974,
Pubmed
,
Echinobase
Grunstein,
Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
1975,
Pubmed
Grunstein,
The histone H4 gene of Strongylocentrotus purpuratus: DNA and mRNA sequences at the 5' end.
1978,
Pubmed
,
Echinobase
Hentschel,
Sea urchin histone mRNA termini are located in gene regions downstream from putative regulatory sequences.
1980,
Pubmed
,
Echinobase
Hnilica,
The specificity of histones in chicken erythrocytes.
1964,
Pubmed
Jeffreys,
A physical map of the DNA regions flanking the rabbit beta-globin gene.
1977,
Pubmed
Kacian,
Synthesis of extensive, possibly complete, DNA copies of poliovirus RNA in high yields and at high specific activities.
1976,
Pubmed
Kedes,
Histone genes and histone messengers.
1979,
Pubmed
Land,
5'-Terminal sequences of eucaryotic mRNA can be cloned with high efficiency.
1981,
Pubmed
Laskey,
Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
1975,
Pubmed
Maniatis,
Nucleotide sequence of the rightward operator of phage lambda.
1975,
Pubmed
Maniatis,
Amplification and characterization of a beta-globin gene synthesized in vitro.
1976,
Pubmed
Maxam,
A new method for sequencing DNA.
1977,
Pubmed
Molgaard,
Histone H5 messenger RNA is polyadenylated.
1980,
Pubmed
NEELIN,
THE HISTONES OF CHICKEN ERYTHROCYTE NUCLEI.
1964,
Pubmed
Payvar,
Improvements in immunoprecipitation of specific messenger RNA. Isolation of highly purified conalbumin mRNA in high yield.
1979,
Pubmed
Perry,
Messenger RNA turnover in mouse L cells.
1973,
Pubmed
Perucho,
An improved method for the preparation of undegraded polysomes and active messenger RNA from immature chicken erythrocytes.
1979,
Pubmed
Renart,
Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: a method for studying antibody specificity and antigen structure.
1979,
Pubmed
Rice,
Radioactive labeling of proteins in vitro.
1971,
Pubmed
Roychoudhury,
Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase.
1976,
Pubmed
Ruiz-Carrillo,
Processing of newly synthesized histone molecules.
1975,
Pubmed
Ruiz-Carrillo,
Selective synthesis and modification of nuclear proteins during maturation of avian erythroid cells.
1976,
Pubmed
Ruiz-Carrillo,
Stability and reversibility of higher ordered structure of interphase chromatin: continuity of deoxyribonucleic acid is not required for maintenance of folded structure.
1980,
Pubmed
Ruiz-Carrillo,
An octamer of core histones in solution: central role of the H3-H4 tetramer in the self-assembly.
1979,
Pubmed
Sanger,
The use of thin acrylamide gels for DNA sequencing.
1978,
Pubmed
Schaffner,
Genes and spacers of cloned sea urchin histone DNA analyzed by sequencing.
1978,
Pubmed
,
Echinobase
Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed
Staden,
Further procedures for sequence analysis by computer.
1978,
Pubmed
Staden,
Sequence data handling by computer.
1977,
Pubmed
Stahl,
Fate of histone messenger RNA in synchronized HeLa cells in the absence of initiation of protein synthesis.
1977,
Pubmed
Sures,
Leader sequences of Strongylocentrotus purpuratus histone mRNAs start at a unique heptanucleotide common to all five histone genes.
1980,
Pubmed
,
Echinobase
Sures,
Identification and location of the histone H2A and H3 genes by sequence analysis of sea urchin (S. purpuratus) DNA cloned in E. coli.
1976,
Pubmed
,
Echinobase
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
Villa-Komaroff,
A bacterial clone synthesizing proinsulin.
1978,
Pubmed
