ECB-ART-33456
J Cell Biol
1972 Oct 01;551:74-81. doi: 10.1083/jcb.55.1.74.
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Selective inhibition of genetic transcription in sea urchin embryos. Incorporation of 5-bromodeoxyuridine into low molecular weight nuclear DNA.
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Experimental evidence suggests that exposure of sea urchin gastrulae to 5-bromodeoxyuridine (BUdR), an analog of thymidine, causes a reduction in the rate of synthesis of some RNA species usually transcribed at this stage of development. In pulse-chase experiments, (3)H (in gastrula stage 8-15S nuclear DNA labeled with BUdR-(3)H) could not be chased, with unlabeled BUdR, into 20-60S size-range DNA; in similar experiments in which gastrulae were pulsed with thymidine-(3)H and then chased with unlabeled thymidine, (3)H in 8-15S nuclear DNA could be extensively chased into 20-60S DNA. DNA-RNA hybridization assays indicate that gastrula stage nuclear DNA in the range of 20-60S has greater sequence homology for gastrula stage RNA-(3)H than does nuclear DNA of similar size-range taken from gastrulae exposed to BUdR for 1 hr. An explanation is offered for the effect of BUdR on transcription and DNA replication in sea urchin embryos.
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Genes referenced: LOC100887844
References [+] :
Abbott,
The loss of phenotypic traits by differentiated cells, V. The effect of 5-bromodeoxyuridine on cloned chondrocytes.
1968, Pubmed