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J Cell Biol
1972 May 01;532:494-509. doi: 10.1083/jcb.53.2.494.
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The ciliary necklace. A ciliary membrane specialization.
Gilula NB
,
Satir P
.
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Cilia, primarily of the lamellibranch gill (Elliptio and Mytilus), have been examined in freeze-etch replicas. Without etching, cross fractures rarely reveal the 9 + 2 pattern, although suggestions of ninefold symmetry are present. In etched preparations, longitudinal fractures through the matrix show a triplet spoke alignment corresponding to the spoke periodicity seen in thin sections. Dynein rows can be visualized along the peripheral microtubules in some preparations. Fracture faces of the ciliary membrane are smooth with few membrane particles, except in the regions adjacent to the basal plate. In the transition region below the plate, a unique particle arrangement, the ciliary necklace, is found. In the Elliptio gill, on fracture face A the necklace is comprised of three well-defined rows or strands of membrane particles that encircle the ciliary shaft. The rows are scalloped and each scallop corresponds to a peripheral doublet microtubule. In thin sections at the level of these particles, a series of champagne-glass structures link the microtubular doublets to the ciliary membrane. The ciliary necklace and this "membrane-microtubule" complex may be involved in energy transduction or the timing of ciliary beat. Comparative studies show that these features are present in all somatic cilia examined including those of the ameboflagellate Tetramitus, sea urchin embryos, rat trachea, and nonmotile cilia of cultured chick embryo fibroblasts. The number of necklace strands differs with each species. The necklace has not been found in rat or sea urchin sperm.
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,
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Bacetti,
The spermatozoon of Arthropoda. 13. The cell surface.
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Branton,
Fracture faces of frozen membranes.
1966,
Pubmed
Branton,
Freeze-etching studies of membrane structure.
1971,
Pubmed
Branton,
Fracture faces of frozen myelin.
1967,
Pubmed
Bretscher,
A major protein which spans the human erythrocyte membrane.
1971,
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Chalcroft,
An interpretation of liver cell membrane and junction structure based on observation of freeze-fracture replicas of both sides of the fracture.
1970,
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Deamer,
Fracture planes in an ice-bilayer model membrane system.
1967,
Pubmed
Eckert,
Passive electrical properties of Paramecium and problems of ciliary coordination.
1970,
Pubmed
Flower,
Particles within membranes: a freeze-etch view.
1971,
Pubmed
GIBBONS,
STUDIES ON THE PROTEIN COMPONENTS OF CILIA FROM TETRAHYMENA PYRIFORMIS.
1963,
Pubmed
GIBBONS,
On flagellar structure in certain flagellates.
1960,
Pubmed
GIBBONS,
The relationship between the fine structure and direction of beat in gill cilia of a lamellibranch mollusc.
1961,
Pubmed
GIBBONS,
REACTIVATION OF GLYCERINATED CILIA FROM TETRAHYMENA PYRIFORMIS.
1965,
Pubmed
Gilula,
The septate junction: a structural basis for intercellular coupling.
1970,
Pubmed
Gilula,
Metabolic coupling, ionic coupling and cell contacts.
1972,
Pubmed
Goodenough,
A fine structural analysis of intercellular junctions in the mouse liver.
1970,
Pubmed
MOOR,
A new freezing-ultramicrotome.
1961,
Pubmed
PORTER,
The submicroscopic morphology of protoplasm.
2002,
Pubmed
Pinto da Silva,
Membrane splitting in freeze-ethching. Covalently bound ferritin as a membrane marker.
1970,
Pubmed
Pinto da Silva,
Localization of A antigen sites on human erythrocyte ghosts.
1971,
Pubmed
Rosenbaum,
Flagellar elongation and shortening in Chlamydomonas. The use of cycloheximide and colchicine to study the synthesis and assembly of flagellar proteins.
1969,
Pubmed
SATIR,
STUDIES ON CILIA. THE FIXATION OF THE METACHRONAL WAVE.
1963,
Pubmed
SILVESTER,
THE CILIA OF TETRAHYMENA PYRIFORMIS: X-RAY DIFFRACTION BY THE CILIARY MEMBRANE.
1964,
Pubmed
STEERE,
Electron microscopy of structural detail in frozen biological specimens.
1957,
Pubmed
Steck,
Disposition of the major proteins in the isolated erythrocyte membrane. Proteolytic dissection.
1971,
Pubmed
Tillack,
Demonstration of the outer surface of freeze-etched red blood cell membranes.
1970,
Pubmed
Wehrli,
Membrane structure as seen with a double replica method for freeze fracturing.
1970,
Pubmed