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Biochem J
1985 Jan 15;2252:429-34. doi: 10.1042/bj2250429.
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ADP-ribosyltransferase in isolated nuclei from sea-urchin embryos.
Isoai A
,
Yasumasu I
.
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The activity of ADP-ribosyltransferase in nuclei isolated from sea-urchin embryos was estimated by the incorporation of [adenosine-14C]NAD+ into the acid-insoluble fraction. Hydrolysis of this acid-insoluble product by snake venom phosphodiesterase yielded radioactive 5''-AMP and phosphoribosyl-AMP. The incorporation of [14C]-NAD+ was inhibited by 3-aminobenzamide and nicotinamide, potent inhibitors of ADP-ribosyltransferase. [14C]NAD+ incorporation into the acid-insoluble fraction results from the reaction of ADP-ribosyltransferase. The optimum pH for the enzyme in isolated nuclei was 7.5. The enzyme, in 50 mM-Tris/HCl buffer, pH 7.5, containing 0.5 mM-NAD+ and 0.5 mM-dithiothreitol, exhibited the highest activity at 18 degrees C in the presence of 14 mM-MgCl2. The apparent Km value for NAD+ was 25 microM. The activity of the enzyme was measured in nuclei isolated from the embryos at several stages during early development. The activity was maximum at the 16-32-cell stage and then decreased to a minimum at the mesenchyme blastula stage. Thereafter its activity slightly increased at the onset of gastrulation and decreased again at the prism stage.
Adamietz,
Rapid determination of chain length pattern in poly (ADP-ribose) samples.
1978, Pubmed
Adamietz,
Rapid determination of chain length pattern in poly (ADP-ribose) samples.
1978,
Pubmed
Albanese,
Unusual properties of sea urchin unfertilized egg chromatin.
1980,
Pubmed
,
Echinobase
BURTON,
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.
1956,
Pubmed
Berger,
Synthesis of DNA and poly(adenosine diphosphate ribose) in normal and chronic lymphocytic leukemia lymphocytes.
1978,
Pubmed
Brightwell,
Poly(adenosine diphosphate ribose) polymerase in Physarum polycephalum.
1975,
Pubmed
Burzio,
A functional role of polyADPR in DNA synthesis.
1970,
Pubmed
Burzio,
ADP ribosylation of rat liver nucleosomal core histones.
1979,
Pubmed
Caplan,
Poly(ADPRibose) levels as a function of chick limb mesenchymal cell development as studied in vitro and in vivo.
1979,
Pubmed
Clark,
Inhibition of nuclear NAD nucleosidase and poly ADP-ribose polymerase activity from rat liver by nicotinamide and 5'-methyl nicotinamide.
1971,
Pubmed
Durkacz,
(ADP-ribose)n participates in DNA excision repair.
1980,
Pubmed
Farzaneh,
The activity and properties of poly(adenosine diphosphate ribose) polymerase in vitro during the embryonic development of the South African clawed toad Xenopus laevis.
1979,
Pubmed
Hasegawa,
The polymerization of adenosine 5'-diphosphate-ribose moiety of NAD by nuclear enzyme. II. Properties of the reaction product.
1967,
Pubmed
Hayaishi,
Poly(ADP-ribose) and ADP-ribosylation of proteins.
1977,
Pubmed
Ito,
Purification and characterization of poly(ADP-ribose) synthetase from calf thymus.
1979,
Pubmed
LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed
Nishizuka,
Studies on the polymer of adenosine diphosphate ribose. I. Enzymic formation from nicotinamide adenine dinuclotide in mammalian nuclei.
1967,
Pubmed
Nishizuka,
Enzymic adenosine diphosphate ribosylation of histone and poly adenosine diphosphate ribose synthesis in rat liver nuclei.
1968,
Pubmed
Ogata,
ADP-ribosylation of histone H1. Identification of glutamic acid residues 2, 14, and the COOH-terminal lysine residue as modification sites.
1980,
Pubmed
Okolie,
ADP-ribosyltransferase in Plasmodium (malaria parasites).
1983,
Pubmed
Purnell,
Novel inhibitors of poly(ADP-ribose) synthetase.
1980,
Pubmed
Riquelme,
ADP ribosylation of rat liver lysine-rich histone in vitro.
1979,
Pubmed
Römer,
Identity of nuclear NAD nucleosidase with a polyADP-ribose forming enzyme in Ehrlich ascites tumor cells.
1968,
Pubmed
Shima,
Studies on poly adenosine diphosphate-ribose. VII. Methods of separation and identification of 2'-(5"-phosphoribosyl)-5'-adenosine monophosphate, ribosyladenosine monophosphate, and phosphoribosyladenosine.
1969,
Pubmed
Stone,
Poly(adenosine diphosphoribose) polymerase in mammalian nuclei. Characterization of the activity in mouse fibroblasts (LS cells).
1973,
Pubmed
Tanuma,
ADP-ribosylation of nonhistone high mobility group proteins in intact cells.
1983,
Pubmed
Tsopanakis,
Purification and properties of poly(ADP-ribose) polymerase from pig-thymus nuclei.
1978,
Pubmed
Ueda,
Natural occurrence of poly(ADP-ribosyl) histones in rat liver.
1975,
Pubmed
Yoshihara,
Evidence for adenosine diphosphate ribosylation of Ca2+, Mg2+-dependent endonuclease.
1975,
Pubmed
